Lecture and laboratory course on principles and techniques of molecular biotechnology. Emphasis on recent techniques in molecular biology. Laboratory exercises include isolation, cloning, and sequencing of genetic material; protein purification; gel electrophoresis of proteins and nucleic acids; DNA synthesis; RFLPs and PCR techniques; construction and screening of DNA and genomic libraries. Industrial applications of molecular biology presented.
Lec. 3 hrs., lab. 3 hrs. Prerequisite: 21:120:355 or 21:120:356 or 21:120:360, with a grade of C or better.
Lab Fee: $100